ricF [2018-03-08 16:37:36]

subunit of the regulatory iron-sulfur containing YmcA-YlbF-YaaT complex, antagonist of biofilm repression by SinR, control of the phosphorelay

Locus: BSU14990

Isoelectric point: 5.01

Molecular weight: 16.77 kDa

Protein length: 149 aa

Gene length: 447 bp

Function: regulation of biofilm formation and the phosphorelay

Product: subunit of the regulatory iron-sulfur containing YmcA-YlbF-YaaT complex

Essential: no

Synonyms: ylbF

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → RNases → Effectors of RNA degradation
• Information processing → Regulation of gene expression → Transcription factors and their control → Control of transcription factor (other than two-component system)
• Information processing → Regulation of gene expression → phosphorelay → Other protein controlling the activity of the phosphorelay
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Other proteins required for biofilm formation
• Lifestyles → Sporulation → phosphorelay → Other protein controlling the activity of the phosphorelay

Gene

Coordinates: 1,568,420 → 1,568,869

Phenotypes of a mutant

• strongly reduced genetic competence, strongly reduced sporulation PubMed

The protein

Catalyzed reaction/ biological activity

• the YmcA-YlbF-YaaT complex stimulates Spo0A phosphorylation in the phosphorelay PubMed

Localization

• cytoplasm PubMed

Expression and Regulation

Operons

• Genes: ylbF
  Description: PubMed

• Genes: ylbF-ylbG-ylbH-ylbI
  Description: PubMed

Biological materials

Mutant

• BKE14990 (ΔylbF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG
• BKK14990 (ΔylbF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG

References

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